Seed Germination Behavior of Dianthus Caryophyllus L.VAR. Chabaud on Treatment of Ethyl Methyl Sulphonate (EMS)
Author : Deshmukh PD and Pundkar SV
Abstract :
The present investigation was carried out to evaluate the effect of different concentration of EMS on seed germination in Dianthus caryophyllus L. var. Chabaud. Seeds were treated with three different treatments viz., 12 Hrs. Dry, 12 Hrs. pre-soaked + 6 Hrs. EMS and 18 Hrs. pre-soaked + 6Hrs. EMS. Different doses of EMS were selected on the basis of LD 50 and doses 0.003%, 0.006% and 0.009% were used for experimentation along with control. Germination was carried out in Petri-plates in controlled condition in three replicas. The study revealed that increased doses concentration results into corresponding decreased in germination percentage for all the three treatments. Treatment 18Hrs. PSW + 6Hrs. EMS shows better germination percentage followed by 12Hrs. PSW + 6Hrs. EMS and 18Hrs. Dry. 18Hrs. Dry EMS showed 76% germination at 0.003% dose, 68% for 0.006% dose and 59% for 0.009% dose as compared to control (81% germination). Slight increase in germination percentage was recorded in 12Hrs. PSW + 6Hrs. treatment. Doses 0.003%, 0.006% and 0.009% showed 79%, 69%, 63% germination in 12Hrs. PSW + 6Hrs. treatment of EMS with control (83%). Highest germination percentage was recorded for 18Hrs. PSW + 6Hrs. treatment in dose 0.003% with 81% germination. It was followed by 0.006% with 73% germination and 0.009% with 65% germination. However control recorded maximum (84%) germination in 18Hrs. PSW + 6Hrs. treatment.
Keywords :
Dianthus caryophyllus, Chabaud, germination, Ethyl Methyl sulphonate (EMS).
