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The Effect of Chia Seed on Pupation Site Preference in Drosophila melanogaster

Author : Afeefa Bhanu, Aneesa PV, Chandana R, Chethan Kumar S and Krishna MS

Abstract :

Pupation site preference (PSP), which includes habitat selection, is an essential part of Drosophila melanogaster preadult development. Larvae's choice of location can have a big impact on how long they survive as pupa. Using three different concentrations of Chia seeds (5g, 10g, and 15g) and wheat-cream agar media (control), the larval pupation site preference (PSP) in D.melanogaster was examined in a lab setting. Our findings demonstrated that PSP varies considerably depending on the concentration. Larvae preferred to pupate on the culture bottle's medium, which were significantly higher in all three concentrations, regardless of concentration. A smaller proportion of larvae chose their pupation site across the wall, and the results varied depending on the medium concentration. The findings indicated that the greatest number of pupations occurred in larvae fed 5 g of chia seed concentration, whereas the least amount of PSP was observed in larvae fed 10 g, 15 g, and wheat cream agar media. Therefore, our research indicates that the nutritious component in the 5 g concentration of chia seeds aids in the larva's pupation on the culture bottle's media. Consequently, our findings demonstrated that the quality and quantity of the nutrients present in different concentrations of Chia seeds can affect Pupation site preference in D.melanogaster.

Keywords :

Pupation site preference (PSP), Diet, D.melanogaster, Pupa, Larva.