Screening and Characterisation of Amylase Producing Bacteria from Azerbaijan Mud Volcanoes Samples
Author : M Raja Rajeswari Bai, I Mani Chandana, N Jakaraiah, B Rajashekar, N Kranthi Kumar, G Swarnalatha and Ch. Chiranjeevi
Abstract :
Soil harbours a diverse range of microorganisms capable of producing industrially significant enzymes, including amylase. Amylase plays a crucial role in various biotechnological applications, such as food processing, pharmaceuticals, and biofuel production. This study focuses on the isolation and characterisation of amylase-producing microorganisms from different soil samples. Soil samples were collected from various ecological niches, including agricultural fields, compost sites, and garden soils. The isolates were screened for amylase production using starch hydrolysis assays. The most potent strains were identified through morphological, biochemical, and molecular techniques. The enzymatic activity was further analysed under varying pH, temperature, and substrate concentrations to determine optimal production conditions. The results demonstrated that several bacterial and fungal strains exhibited significant amylase activity, with Bacillus species. being the predominant producers. This study highlights the potential of soil microorganisms as a valuable source of industrial amylase and their possible applications in biotechnology.
Keywords :
Amylase, Soil Microorganisms, Bacillus, Starch Hydrolysis, Enzyme Production, Biotechnology.
