Study of IHC Expression of Ki-67 and P53 in Phyllodes Tumor in Correlation with Its Histological Grading
Author : Dr. Saloni Patra and Dr. Surekha Bhalekar
Abstract :
The incidence of phyllodes tumor is 1.5% and it is said to be 1 in 100,000 women which makes it a rare fibroepithelial neoplasm, as per other olden studies. The peak of phyllodes tumor occurs in women aged 45 to 49 years. Phyllodes tumor (PT) is basically a biphasic breast tumor that is composed of cellular spindle stroma with epithelial elements but the stromal element is regarded as the neoplastic component. Accurate and reproducible grading of these tumors into benign, borderline and malignant is challenging, and by the virtue of the importance of assessment of multiple histological parameters, certain markers contribute to a streamlined diagnosis.
The aims and objectives are to study the IHC expression of Ki-67 and p53 in phyllodes tumor in correlation with its histological grading, calculate the frequency of benign, borderline and malignant lesion of phyllodes tumor. The study can also determine the association of Ki67 and p53, grade and stage the tumors for therapeutic and prognostic purpose. This is retrospective and a prospective study of breast lesions conducted in a tertiary care hospital from 2017 to 2022 over a period of 60 months with sample size of 30 cases. The study included 11 benign cases, 9 borderline cases, and 10 malignant cases. Expressions of Ki-67 and p53 were evaluated on all the cases and compared in these three categories. We found the mean p53 and Ki67 increased significantly from benign to malignant lesions. In our study, we observed a wide range in age distribution which was between 20-64 years with mean age of 46.4±10.8years. Majority of phyllodes tumor patients fell between 41 to 50 years. A positive correlation was noted between cases with high p53, high Ki 67 and rate of recurrence in the lesion.
Keywords :
Immunohistochemistry, phyllodes tumor, benign, borderline, malignant
