Phytochemical Screening and In Vitro Thrombolytic Activity of Honey of Indian Stingless Honeybee Tetragonula Iridipennis
Author : Hemant B Zope, Dipali G Wankhade, Namrata G Mahajan, Deveshri S Sonawane and Manojkumar Z Chopda
Abstract :
Atherothrombosis is the main cause of cardiovascular diseases. Cardiovascular diseases (CVDs) take the lives of 17.7 million people every year, 31% of all global deaths due to the Heart attacks and strokes caused by a vessel blockage by thrombus. Thrombolytic drugs are available in market, however, they cause some side effects and expensive. Recently, Meliponiculture in India has become popular because the honey has a unique taste, a mix of sweet, sour and bitter which is highly valued. Therefore, present research work carried out to investigate the phytochemical compositions of stingless bee honey and to evaluate In vitro thrombolytic activity of honey of stingless honeybee (Tetragonula iridipennis) using plasma clot method. Clot lysis is check at two concentrations of honey against the artificially prepared plasma clot. Streptokinase used as standard and sterilized distilled water used as control. The percentage of clot lysis was analysed by computing the difference between weights of clot. The test honey at concentration 1ml/10ml and 1ml/5ml showed 9.958 ± 0.860% and 4.397 ± 0.452% clot lysis respectively. Our findings showed that stingless bee honey contains varied phytochemicals-tannins, alkaloids, flavonoids, terpenoids, carotenoids, coumarins, saponins. The stingless bee honey has the potential to dissolve clot, therefore, it is urgent need to isolate the active molecule which is therapeutically beneficial in future use.
Keywords :
Thrombosis, Stingless honeybee, Tetragonula Iridipennis, phytochemicals, thrombolytic activity
