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Biochemical Characterization of Actinomycetes Isolated from Different Soil Samples

Author : Sowmya K L and Dr. Ramalingappa B

Abstract :

In the present study, the different soil samples were collected around Davangere city. Actinomycetes strains were isolated in specific media like Starch Casein Nitrate Agar Medium (SCA), Benedict Agar Medium (BA), Glucose Asparagine Agar Medium (GAA), Oat Meal Agar Medium (OMA). Cultural characteristics of actinomycetes are determined based on morphology, production of diffusible pigments, utilization of various carbon and nitrogen sources and by various physiological tests. Actinomycetes strains were performed in biochemical tests such as Fermentation of citrate, starch hydrolysis, triple sugar iron agar, gelatine hydrolysis, casein hydrolysis etc. Strategy for a range of biochemical characterization methods have been mentioned in this article for the discovery of various genera of actinomycetes.

Keywords :

Actinomycetes, Biochemical tests, Streptomyces sp, Nocardia sp. Saccharopolyspora sp, Amycolatopsis sp