Development and Evaluation of Novel Drug Delivery System of Hydroalcoholic Extract of Alhagi Camelorum against Paracetamol Induced Hepatotoxicity in Rats
Author : Rishi Kant Tripathi, Dr. Narendra Singh and Dr. Pritt Verma
Abstract :
Despite use of Alhagi camelorum extract against liver disorders, the clinical use of it is limited due to their poor solubility and instability. Moreover, the available therapies are facing the challenges of efficacy and safety. Hence, Alhagi camelorum extract loaded nanoemulsion has been formulated to improve its hepatoprotective activity. The animals were segregated in 6 groups (6 male rats each) weighing 250-290 g. Group I animals were treated with normal saline, Group II animals were treated with paracetamol at the dose of 2g/kg while Group III were treated with Alhagi camelorum hydroalcoholic extract at the dose of 400mg/kg, Group IV and V were the test formulation and were treated at the dose equivalent to 400mg/kg and half dose or equivalent dose to 200mg/kg. Group VI was treated with standard silymarin at the dose of 25 mg/kg for seven consecutive days, respectively. On 5th day 30 minutes after respective treatments, paracetamol 2g/kg orally was administered. After seven consecutive days, blood serum samples and liver tissues were collected for biochemical and histopathological analysis. Phytochemical screening was carried out to screen for phytochemical classes and HPLC analysis was conducted to screen polyphenols. The administration of AC extract showed hepatoprotection at the doses of 400 mg/kg. However, nanoemulsion loaded AC (dose 400 mg/kg) significantly reduces the elevated serum levels of liver biomarkers compared to the paracetamol-induced hepatotoxic group. These findings were confirmed with histopathological changes where nanoemulsion was capable of reversing the toxic effects of paracetamol acid on liver cells.
Keywords :
Alhagi camelorum, novel drug delivery system, nanoemulsion; hepatoprotection, particle size
